Lezaun A, Fraj J, Colas C, Duce F, Dominguez MA, Cuevas M, Eiras PĪlonso L, Marcos M L, Blanco JG, Navarro JA, Juste S, del Mar Garcés M, Perez R, Carretero PJĪnaphylaxis caused by linseed (flaxseed) intake. 2003Ī report of six cases of flaseed sensitization with review of the literatureįood skin testing in patients with idiopathic anaphylaxis. IgE cross-reactivity and PolysensitisationĪllergy to linseed was one of the first food allergies described in the modern literature (Black 1930, Grant 1931 ). Dose response: No oral provocation data published.Number of Patients: No oral provocation data published.Blind: No oral provocation data published.Food used and oral provocation vehicle: No oral provocation data published.It was suggested that the 22kD protein was the main allergen (Alonso et al 1996 ). IgE from patient bound to five protein bands 20, 22, 30, 35 and 38kDa. Immunoblot showed allergens of 150-175 and 90-100 kDa before reduction and 35-100 kDa after reduction. (2002) suggest that the allergen is a dimer. One major allergen was identified before reduction running at 56 kDa which ran at 28 kDa after reduction. Immunoblotting detection method: Proteins were transfered to PVDF membranes and IgE binding was revealed with goat anti-human IgE (Leon et al.Samples without reducing agent and mercaptoethanol reduced samples were loaded at 40 µg/lane. (2002) used 1D SDS PAGE with a 10% separating gel. Immunoblotting separation: Leon et al.Linseed specific IgE was 20kU/L with a total of 221 kU/L Leon et al. Linseed specific IgE was 2.16 kU/L with a total of 19 kU/L (Alonso et al 1996 ). Number of Patients: Single cases are reported by Alonso et al (1996) Lezaun et al.(2003) defatted the linseed with acetone before extracting with phosphate buffered saline. Extract sterile-filtered (Alonso et al 1996 ). One patient gave a positive scratch test to proteins from flax (Stickler et al 1986 ).ĥg of linseed incubated in 50ml phosphate buffered saline for 24 hours at 4C, with stirring. This was considered positive although the negative control was 6 mm diameter (Alonso et al 1996 ). The patient reacted with 14x14mm wheal (Lezaun et al. The patient reacted with 5x4mm wheal (Leon et al. Single cases are reported by Strickler et al (1986) Alonso et al (1996) Lezaun et al. The positive control was histamine, 10 mg/ml, and the negative control was phosphate-buffered saline (Alonso et al 1996 ). Protocol: (controls, definition of positive etc) Prick-Prick test with linseed.Tingling and burning sensation of lips and tongue, swollen tongue and throat, vomiting, conjunctivitis and conjunctival and eyelid oedema, scratch test positive to proteins from flax in one patient, same symptoms and generalised urticaria in another (Black 1930 ). Episodes occurred 2-3min after eating multigrain bread (Lezaun et al 1998 ).Īnaphylactic reaction after one spoonful of linseed oil: ocular pruritis, weeping, strong palmar pruritis, generalised urticaria, nausea and vomiting (Alonso et al 1996 ).Īnaphylaxis in one patient (Strickler et al 1986 ). Several episodes of anaphylaxis manifested as intestinal/abdominal pain, vomiting, diarrhoea, generalised urticaria, acute dyspnea, pruritis, sneezing, nasal obstruction, general malaise which required emergency treatment. Anaphylaxis after eating a spoonfull of grains of linseed (Leon et al.
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